Journal: bioRxiv

Article Title: Increased utrophin expression in healthy and DMD patient derived myoblasts in response to ERK1/2 and EZH2 inhibitor treatment

doi: 10.64898/2026.04.13.718206

Figure Lengend Snippet: Corresponding to . a . Basal levels of MYOD1 mRNA in primary healthy human myoblasts (n=6) and primary DMD patient-derived myoblasts (n=16). Lines show median and quartiles. b . AB1098 cells were treated with 10 μM LY32 +/- 10 μM GSK503 or vehicle (DMSO) for 24 hours. Directly following treatment (0 days since drug removal) cells were manually counted, with trypan blue staining to discount dead cells. Cells were then replated at a constant concentration, cultured in proliferation media without drugs for 24 hours and counted again (1 day since drug removal). Cells were then replated at a constant concentration and cultured again for 6 days before counting (7 days since drug removal). Cells were passaged once in this 6-day period. Cell count is shown relative to the vehicle only control for each time point. c . RT-qPCR of MYOD1 in immortalised myoblasts AB1190 and AB1098 following treatment with varying concentrations of Ruxolitinib (Ruxo). Results are shown relative to vehicle treated sample with a two-way ANOVA with Dunnetts multiple comparison test to determine significance. Graph shows mean (n=3) +/- SEM. a-b . MYOD1 is normalised against TBP . *p<0.05, ***p<0.001, ****p<0.0001.

Article Snippet: The muscle biopsy was enzymatically digested in basal medium (C-23060; PromoCell, Heidelberg, Germany) containing collagenase D (2 mg/mL, Roche, Germany) and Dispase II (2 mg/mL, Sigma) for 1 h at 37°C with trituration every 15 min. After filtering through a 100-μm filter (BD Falcon) and centrifugation cells were resuspended in proliferation medium (15% FBS, C-23060; PromoCell, Heidelberg, Germany) and transferred to a T-25 tissue culture vessel (Nunc, Germany).

Techniques: Derivative Assay, Staining, Concentration Assay, Cell Culture, Cell Characterization, Control, Quantitative RT-PCR, Comparison

Journal: Journal of Materials Chemistry. B

Article Title: Systematic investigation of the effects of neural stem cell spheroid size and density on fate specification in 3D culture

doi: 10.1039/d5tb01589h

Figure Lengend Snippet: On day 5 of culture in differentiation medium, the hydrogels were fixed, sectioned at 10 µm and stained for (a) Ki-67 and (b) Ki-67 with Hoescht to confirm the presence of proliferative cells. Scale bars are 100 µm. (c) These images were analyzed using cell profiler to determine the percentage of nuclear expression (* p < 0.05, N = 11, error bars represent ± SEM). A test of normality (Shapiro–Wilk test) and subsequently ordinary two-way ANOVA (Tukey's multiple comparison test) was performed using GraphPad Prism to determine significance difference between the groups. The small sphere, low density group had a higher proportion of nuclear Ki-67 expression than the small sphere, high density group. (d) Flow cytometry was performed to compare proliferation, represented by the percentage of EdU positive cells. A test of normality (Shapiro–Wilk test) and subsequently ordinary two-way ANOVA (Tukey's multiple comparison test) was performed using GraphPad Prism to determine significance between the groups. ( N = 3–4, error bars represent ± SEM). No significant differences were observed.

Article Snippet: They were then cultured in these plates with proliferation medium (0.02 μg mL −1 epidermal growth factor (Fisher Scientific, AF10015100), 0.02 μg mL −1 basic fibroblast growth factor (ThermoFisher Scientific, 100-18B-100UG), 2 μg mL −1 heparin (Millipore Sigma, H6279-25KU) and 0.2× ABAM (Millipore Sigma, A5955-20 mL) in basal medium (Millipore Sigma, SCM003)) for 48 hours in an incubator (37 °C, 5% CO 2 ).

Techniques: Staining, Expressing, Comparison, Flow Cytometry

Journal: Materials Today Bio

Article Title: Neuroactive network tissue based on dual-factor neuroregenerative bioactive coating scaffolds and neural stem cells for spinal cord injury repair

doi: 10.1016/j.mtbio.2025.102172

Figure Lengend Snippet: Preparation and bioactivity evaluation of immobilized recombinant growth factors and the construction of the neuroregenerative coating fiber network scaffold. (A) SDS-PAGE gel showed the preparation process of immobilized recombinant growth factors D-IGF1 and D-NGF including: marker (M), pre-induction (1), post-induction (2), supernatant after centrifugation (3), pellet after centrifugation (4), refolded protein solution (5), flow-through liquid (6), wash liquid (7), elution liquid (8). (B) The effect of different densities of D-IGF1 and D-NGF on NSC proliferation. (C–D) The effect of different densities of D-IGF1 and D-NGF on neural stem cell differentiation. Scale bars, 100 μm (E) SEM images of the surface morphology of fiber scaffolds prepared under different process parameters. Scale bars, 10 μm (F–G) Biocompatibility testing of the scaffold. (H) Contact angle analysis. (I) FT-IR analysis. (J) XPS analysis. (Data are shown as the mean ± SD, n = 3, ∗p < 0.05,∗∗p < 0.01).

Article Snippet: The resulting cells were resuspended in NSC proliferation medium containing DMEM/F12 (Gibco, A4192001, USA), 1 % penicillin-streptomycin (PS, Sigma-Aldrich, USA), 1 % glutamine (Gibco, USA), 20 ng mL −1 basic fibroblast growth factor (bFGF, Solarbio, China), and 20 ng mL −1 epidermal growth factor (EGF, Solarbio, China).

Techniques: Recombinant, SDS Page, Marker, Centrifugation, Cell Differentiation